MiniProfiler MP4 System

Purpose Short-term temporal variability of microphytobenthic primary production is suspected to be of the same magnitude as seasonal variability, but data remain very scarce due to methodological limitations. In this context, a 6-day in situ high-frequency survey was performed in a temperate intertidal mudflat using an automated microprofiling system. Materials and methods In situ microphytobenthic primary production was measured using an automated acquisition system for oxygen microprofiles. More than 900 microprofiles, acquired during six consecutive days in April 2008, allowed the establishment of robust relationships between oxygen production and irradiance. Moreover, simultaneous measurements of fluorescence parameters and oxygen microprofiles during two diurnal emersion periods led to significant correlations between relative electron transport rate (rETR) and gross oxygen production (GOP). Results and discussion The use of an automated system allowed the estimation of oxygen exchanges during both immersion and emersion periods, and to our knowledge, this is the first study presenting continuous measurements during six consecutive days. The intertidal mudflat studied here was characterized by a maximum net oxygen production of 6.74 mmol O2 m?2 h?1. Evidence for microphytobenthic migration behavior was observed during several periods and induced important depletion in oxygen production while irradiance remained high. Consequently, estimations of GOP from fitted photosynthesis–irradiance curves (P–I curves) showed an overestimation of 31 % compared to the GOP actually measured during the whole deployment. Conclusions This study confirmed that oxygen microsensors may be used to record microphytobenthic primary production, as resulting dynamics agreed with fluorescence data, while production values were in accordance with those presented in the literature. High-frequency microprofiles acquisition may be an easy way to monitor short-term temporal changes in microphytobenthic primary production in order to calculate accurate carbon or oxygen budgets for intertidal areas.

A study of organic carbon mineralization from the Congo continental shelf to the abyssal plain through the Congo submarine channel and Angola Margin was undertaken using in situ measurements of sediment oxygen demand as a tracer of benthic carbon recycling. Two measurement techniques were coupled on a single autonomous platform: in situ benthic chambers and microelectrodes, which provided total and diffusive oxygen uptake as well as oxygen microdistributions in porewaters. In addition, sediment trap fluxes, sediment composition (Org-C, Tot-N, CaCO3, porosity) and radionuclide profiles provided measurements of, respectively input fluxes and burial rate of organic and inorganic compounds. The in situ results show that the oxygen consumption on this margin close to the Congo River is high with values of total oxygen uptake (TOU) of 4±0.6, 3.6±0.5 mmol m-2d-1at 1300 and 3100 m depth, respectively, and between 1.9±0.3 and 2.4±0.2 mmol m-2d-1at 4000 m depth. Diffusive oxygen uptakes (DOU) were 2.8±1.1, 2.3±0.8, 0.8±0.3 and 1.2±0.1 mmol m-2d-1, respectively at the same depths. The magnitude of the oxygen demands on the slope is correlated with water depth but is not correlated with the proximity of the submarine channel-levee system, which indicates that cross-slope transport processes are active over the entire margin. Comparison of the vertical flux of organic carbon with its mineralization and burial reveal that this lateral input is very important since the sum of recycling and burial in the sediments is 5-8 times larger than the vertical flux recorded in traps. Transfer of material from the Congo River occurs through turbidity currents channelled in the Congo valley, which are subsequently deposited in the Lobe zone in the Congo fan below 4800 m. Ship board measurements of oxygen profiles indicate large mineralization rates of organic carbon in this zone, which agrees with the high organic carbon content (3%) and the large sedimentation rate (19 mm y-1) found on this site. The Lobe region could receive as high as 19 mol C m-2y-1, 1/3 being mineralized and 2/3 being buried and could constitute the largest depocenter of organic carbon in the South Atlantic. © 2009 Elsevier Ltd. All rights reserved.

An Integrated Sediment Disturber (ISD) was developed to study the effects of repeated physical disturbances on sediment geochemistry and associated benthic fauna. The ISD is capable to generate a strong, more or less uniform mixing of surface sediments by three rotating fork-like disturber units of approximately 1 m in diameter. Perturbations can be set at different intensities and frequencies, e. g., on times scales of hours, days, or weeks, depending on the experimental design. The ISD carries an Autonomous Positioning Drive (APD) module, which allows the three-dimensional positioning of sensors by a programmable controller. The three circular disturbed zones and an undisturbed control area in-between can be monitored at specified frequencies using a microprofiler with microelectrodes for measuring various parameters (e. g., oxygen, pH, hydrogen sulphide). All operations are supervised by a digital stills camera. Sediment sampling at the start and end point using coring devices handled by divers (and potentially undertaken using a Remotely Operated Vehicle) provides material to study shifts in biodiversity of the small sediment-inhabiting biota and alterations in ecosystem functioning in response to perturbations and the changing geochemical characteristics of surface sediments.