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Biofilm - O2 and N2O microprofiles in samples from CF patients

O2 and N2O microprofiles in sputum samples from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection

With the Unisense microsensors and the Unisense MicroProfiling System you can complete microprofiles in biofilms with extreme positioning accuracy (precision < 10 μm) and high spatial resolution to obtain valuable information about the microenvironment.

Kolpen et al. (2014) used the Unisense O2 and N2O microsensors to measure microprofiles in sputum samples from cystic fibrosis patients with chronic Pseudomonas aeruginosa infection. P. aeruginosa is the major cause of chronic lung infection of cystic fibrosis patients where the bacteria live as biofilm aggregates in the lungs. The biofilms can persist for years in the airways of the patient despite an active immune response and antibiotic therapy. The measurements in the paper by Kolpen et al. provided new insights about the microenvironment and growth of the P. aeruginosa biofilm which may lead to new treatment strategies.

Based on the O2 and N2O microsensor profiling measurements, the authors demonstrated that sputum samples from patients with chronic P. aeruginosa infection consist of an upper oxygenated zone and a lower anoxic zone below around 3 mm from the sputum surface (Figure 2). N2O production from the bacteria was mainly confined to the lower anoxic part and a maximum median concentration of 41.8 μM N2O was found. Significantly less N2O was found in control sputum samples from cystic fibrosis patients without infection. N2O is an intermediate in the denitrification pathway and the data indicated that P. aeruginosa may acquire energy for growth from denitrification when O2 is absent. Using the Unisense microsensors to obtain O2 and N2O concentration gradients with high spatial resolution, the authors could explore the microenvironment in the sputum and they demonstrated N2O production in clinical samples from infected cystic fibrosis patients for the first time.

Please read the full application note. The results are published in Kolpen et al. (2014) Nitrous oxide production in sputum from cystic fibrosis patients with chronic pseudomonas aeruginosa lung

 

Suggested Products
Sensors OX-25, N2O-25
Amplifier System Microsensor Multimeter
Positioning Equipment MicroProfiling System
Software SensorTrace Suite (including Profiling software)

 

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