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Respiration of individual copepod eggs

Copepod eggs can survive extended exposure to high levels of hydrogen sulfide when they become buried in coastal marine sediments. Few higher organisms can survive under these conditions and the preservation mechanism used by the eggs is unknown.

It has not previously been possible to measure the respiration rate of individual eggs. However, a research group at Roskilde University Center, Denmark, lead by Dr. Benni Hansen has made the first respiration rate measurements with Unisense NanoRespiration System

Zoom in on the NanoRespiration System, the stereo microscope is required for correct positioning of the microsensor into the roset...
Schematic drawing of rosette capillary with organism and the measured oxygen concentration profile. 
The central feature of the NanoRespiration is the tiny glass capillaries that constitutes the rosette. Intelligent design for easy...
Screen dump from the Unisense Profile analysis spreadsheet with data from copepod respiration measurements.
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Oxygen profiles were measured in wells containing one, three, and 10 eggs. From these profiles, oxygen respiration rates were calculated. These calculations and plottings are automated by the Unisense Profile Analysis program.

Toxicity studies
High-resolution on-line measurements enable researchers to follow the development in respiration rate during the incubation period. This allows the researcher to evaluate the metabolic effect of adding toxic substances to the assay organism. The monitoring can be performed with a very high temporal resolution.

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NanoRespiration System

Nielsen, Pernille et al (2007), Respiration rates of subitaneous eggs from a marine calanoid copepod: Monitored by nanorespirometry, Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 287 - 296, vol. 177 Read abstract

Lopes, Ana S. et al (2005), Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system, Reproduction, 669 - 679, vol. 130 Read abstract

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