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MicroRespiration System


Measure O2, H2, H2S, N2O, NO, pH, Redox and temperature metabolism in sealed systems

The MicroRespiration System is an excellent tool to measure minute analyte changes in closed chambers. This could be respiration rates of phyloplankton, H2S production in macrophages or pH changes in bacterial suspensions. The two central features of the MicroRespiration System are gas tight glass chambers and protected microsensor.

  • Measurement of two analytes simultaneously
  • Oxygen MicroOptode sensor with automatic temperature compensation
  • View metabolic rates online and in real time
  • Sealed chambers in pressure equilibrium with environment
  • All materials in contact with sample are autoclavable
  • Protected stirring of fragile samples
  • Excellent for teaching purposes
MicroRespiration Chambers come in many different sizes. From left 400 µL, 500 µL, 750 µL, 1 mL, 2 mL, 4 mL. 
Stirring of fragile samples is made possible with a small net separating the magnet from the sample. 
The MicroRespiration double chamber allows for continuous measurement with two MicroRespiration sensors.  
The MicroRespiration Injection Lid has an extra port for injection of substances to your sample - for 4mL chambers only.
For measurement of larger samples the MicroRespiration chambers are available in 20/50 mL and 200/400 mL sizes.
MicroRespiration 0-calibration chamber. Intelligent design for easy calibration of the O2 MicroRespiration sensor.  ...
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MicroRespiration Chambers
The glass chambers are designed to be completely sealed when immersed in water or sealed with oil. Samples are placed in the chambers and the analyte concentrations are measured through a thin capillary in the chamber lids with the MicroRespiration microsensor. The chambers are made in volumes from 400 µL to 400 mL. In addition to this you can have double chambers for measurement of two analytes simultaneously and injection lids to inject substrates to the samples while measuring.

O2 microsensor for MicroRespiration System. 
Temperature microsensor for MicroRespiration System. 
H2 microsensor for MicroRespiration System. 
H2S microsensor for MicroRespiration System. 
NO microsensor for MicroRespiration System. 
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MicroRespiration Microsensors
The MicroRespiration microsensors have insignificant analyte consumption and a very low detection limit that allows for detection of minute concentration changes in the chambers. Correct insertion of the microsensor through the capillary is secured by the aluminum guide protecting the microsensor.

The sensor is guided directly into the capillary hole of the MicroRespiration Chamber. 
MicroRespiration Rack for easy positioning if the MicroRespiration sensor.
Changing chamber size is easy with the new rack chamber fitters. 
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MicroRespiration Setup
The aluminum guide fits perfectly into the MicroRespiration Rack, which holds up to eight chambers (< 4mL). The MicroRespiration Rack guides the microsensor directly into the chambers making the MicroRespiration System easy to use and excellent for teaching purposes. The MicroRespiration Rack has separate stirring of the chambers ensuring homogeneity in the samples. Even fragile samples can be stirred due to a metal net separating the sample from the magnet.

SensorTrace RATE

SensorTrace Rate
The matching software SensorTrace Rate provides real-time and online PC inspection of data and enables measurement and calculation of analyte respiration and metabolism rates in several samples simultaneously. This software allow you to measure and analyze multiple analytes and multiple samples.

Microsensor Amplifier
With the Unisense Amplifier portfolio you can choose any MicroRespiration microsensors you wish for either single analyte or multiple analyte studies.

Ordering information


System components Product
Sensor All Unisense MR-Sensors and Electrodes
Amplifier Opto-F1 or Opto-F4 UniAmp
Single or MultiChannel UniAmp
MicroRespiration Chambers MR-Ch Small (400-4000 µL)
MR-Ch Medium (20-50 mL)
MR-Ch Large (200-400 mL)
Other MR-Chambers MR-Ch 0-calibration (zero calibration)
MR-Ch injection (with injection lid - 4mL chamber only)
MR-Ch double port (for two sensors)
Software SensorTrace Rate
Rack MR2-Rack (for 8 MR-chambers)
Stirring MR2-CO (Stirrer controller)
Magnets (Set of 4 glass magnets)
Calibration Chamber CAL300




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Brodersen, Klaus Peter et al (2008), Respiration of midges (Diptera; Chironomidae) in British Columbian lakes: Oxy-regulation, temperature and their role as palaeo-indicators, Freshwater Biology, 593 - 602, vol. 53 Read abstract

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Sommer, S. et al (2010), Benthic respiration in a seep habitat dominated by dense beds of ampharetid polychaetes at the Hikurangi Margin (New Zealand), Marine Geology, 223 - 232, vol. 272 Read abstract

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