Measure respiration rates of small samples
With the MicroRespiration Systems it is possible to measure the respiration of very small organisms in closed micro systems. The MicroRespiration System is based on use of only one sensor to measure in several micro systems during one experiment. This is possible because the MicroRespiration microsensor is easily moved between the individual MicroRespiration Chambers. The software solution for MicroRespiration called SensorTrace RATE keeps track of the oxygen metabolism in each chamber and interpolates between measurements, hereby giving continuous measurements in each chamber even though the sensor in fact has been moved between the different chambers.
The examples below illustrate the use of the micro-respiration system for biological applications.
Respiration rate of non-biting Midges
The oxygen uptake of aquatic larvae of non-biting midges in the range from atmospheric saturation of oxygen (100% saturation or 21kPa) and down to zero oxygen was investigated using the Unisense MicroRespiration System. The picture above shows a non-biting midget placed in a small MicroRespiration Chamber. The sample can be stirred without damaging of the midge due to the metal net separating the glass coated magnet and the midget.
Respiration rates of individual fish eggs
Respiration rates of individual fish eggs (and other fragile samples) can also be measured with the micro-respiration system. By placing a metal net on top of a glass ring in the chamber the fragile egg can be separated from the rotating magnet. The graph abve shows the respiration of a single fish egg from the Zebra fish.
H2S production rate of macrophages
Zhu et al (2010) used the MicroRespiration System to determine the H2S production rate of cultured macrophages. Cell pellets of cultured macrophages were placed in MicroRespiration Chambers and the H2S production was measured. To avoid spontaneous H2S oxidation in the MicroRespiration Chambers Zhu et al used nitrogen to deoxygenate the culture media in the chambers. H2S production was stimulated by adding L-cysterine and pyridoxal-5’-phosphate to the chambers.