You are here: Applications bread Selected Applications bread Photosynthetic biofilms

Photosynthetic biofilms

Photosynthetic biofilm

O2, H2S and pH microprofiles in biofilm

Microbial mats undergo daily cycles that are characterized by dramatic changes in oxygen, pH and H2S dynamics. The mats are highly active systems with functional layers of only a few hundred microns. To investigate the dynamics within these layers it is important that measurements in the mat can be performed at high spatial resolution. Unisense microsensors can be made with sensor tips down to 3 µm and they are therefore very suited for studies of active systems.
This is a study example of microprofiling completed in a costal sediment mat collected at Aggersund, Denmark. During the measurements the mat was exposed to varying light intensities or darkness. Microsensors were mounted on a motorized MicroProfiling System, microsensor position was monitored using a dissection microscope and sensor data was logged using SensorTrace PRO.

pH_and_H2S_screen_dump The microbial mat was dominated by Cyanobacteria and in light conditions there was a pronounced oxygen production in the photic zone, where a peak in pH was also evident. H2S was found in the anoxic layer below 1200 µm. The marked shifts in dynamics moving from light to dark conditions were captured with the Unisense microsensors. In the dark the H2S production zone moved upwards along with a rapid decline of oxygen production in the microbial mat. Fig.ox-dynamics-both-figures

Suggested Products:
Sensors

OX-25, pH-25, RD-25, H2-25, Ref-RM

Amplifier System Microsensor Multimeter
Positioning Equipment

MicroProfiling System

Software SensorTrace PRO

 

Related products

Microsensors and electrodes

Woelfel,J. et al (2009), Oxygen evolution in a hypersaline crust: photosynthesis quantification by in situ microelectrode profiling and planar optodes in incubation chambers, Aquatic Microbial Ecology, - , vol. 10 Read abstract

Behrendt,L. et al (2011), Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella, The ISME Journal, - , vol.

Tankere,S.P.C. et al (2002), Microenvironments and microbial community structure in sediments, Environmental Microbiology, 97 - 105, vol. 4 Read abstract

Krawczyk-Bärsch,E. et al (2008), Influence of uranium (VI) on the metabolic activity of stable multispecies biofilms studied by oxygen microsensors and fluorescence microscopy., Geochimica et Cosmochimica Acta, 5251 - 5265, vol. 72 Read abstract

Behrendt,L. et al (2012), Biofilm Growth and Near-Infrared Radiation-Driven Photosynthesis of the Chlorophyll d-Containing Cyanobacterium Acaryochloris marina, Applied and Environmental microbiology, 3896 - 3904, vol. 78

See all publications