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Gut Microbiology

P1070465_cr_Andreas_Brune_900x430

Microsensors in the gut of cockroaches

The fine tipped microsensors from Unisense allowed Schauer et al (2012) to investigate and characterize the physicochemical conditions in the gut of the cockroach Shelfordella lateralis.

The research team completed axial microprofiles for oxygen, hydrogen, pH and redox potential directly into intact guts of Shelfordella lateralis. The dissected guts were fixed on a layer of agarose (O2, Redox and H2) or embedded in agarose directly after dissection (pH). The microsensors were mounted on a MM33 and the position of the sensor tip was monitored using a stereo microscope.

Termite-gut-set-up-close-up

Based on the microsensor data, Schauer et al (2012) concluded that the gut of Shelfordella lateralis had an anoxic and reducing environment at the center of all gut compartments and that pH slightly increased from proximal to distal compartments. Furthermore, the researchers revealed that in five out of eight individuals, hydrogen was found in low concentrations throughout the gut, whereas in three of the eight individuals hydrogen strongly accumulated in the midgut and hindgut.

Fig1.cockroaches_Andreas_Brune_900x430

 

Unisense Product List:
Sensors

OX-10
pH-10
Redox-10
H2-10
Ref-100

Amplifier System Microsensor Multimeter
Positioning Equipment

MicroProfiling System

Software SensorTrace PRO

Related products

Microsensors and electrodes

Schauer,C. et al (2012), The bacterial community in the gut of the Cockroach Shelfordella lateralis reflects the close evolutionary relatedness of cockroaches and termites, Appl.Environ.Microbiol., 2758 - 2767, vol. 78 Read abstract

Kohler,T. et al (2012), High-Resolution Analysis of Gut Environment and Bacterial Microbiota Reveals Functional Compartmentation of the Gut in Wood-Feeding Higher Termites (Nasutitermes spp.), Appl.Environ.Microbiol., 4691 - 4701, vol. 78 Read abstract

Brune,A. et al (1995), The Termite Gut Microflora As An Oxygen Sink - Microelectrode Determination of Oxygen and Ph Gradients in Guts of Lower and Higher Termites, Applied and Environmental microbiology, 2681 - 2687, vol. 61 Read abstract

Brune,A. et al (1996), pH profiles of the extremely alkaline hindguts of soil-feeding termites (Isoptera: Termitidae) determined with microelectrodes, Journal of Insect Physiology, 1121 - 1127, vol. 42 Read abstract

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