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NanoRespiration System

NanoResp

Measure minute oxygen consumption rates on small organisms

With the NanoRespiration System you can measure respiration rates on single individuals such as copepod eggs, phyloplankton or zebrafish eggs. The system has high accuracy and respiration rates down to 0,7 fmol/s can be determined.

  • Determine extremely low oxygen consumption rates
  • Fast and repetitive measurements of several samples
  • Accurate and non-destructive measurements by Unisense O2 microsensor
  • Autoclavable capillaries

The NanoRespiration System requires exact positioning and moving of the microsensor tip. This is ensured by integration of the NanoRespiration System into the Unisense MicroProfiling System. Easy operation of the The MicroProfiling System and data logging is done with SensorTrace Profiling and oxygen respiration rates are calculated using a free spread sheet.

Zoom in on the NanoRespiration System, the stereo microscope is required for correct positioning of the microsensor into the roset...
Schematic drawing of rosette capillary with organism and the measured oxygen concentration profile. 
The central feature of the NanoRespiration is the tiny glass capillaries that constitutes the rosette. Intelligent design for easy...
Screen dump from the Unisense Profile analysis spreadsheet with data from copepod respiration measurements.
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NanoRespiration Technology:

Samples are placed in oxygen impermeable and chemical inert glass capillaries with sealed bottoms. Each sample is overlaid with appropriate medium and when the setup has reached steady state an oxygen gradient will build up in the thin capillary. This gradient is measured by a very fine-tipped Unisense microsensor, and the respiration rate of the microorganism placed in the capillary can be calculated from the oxygen gradient.

The NanoRespiration System was developed for determining oxygen respiration rates; however the system is compatible with all Unisense microsensors. In 2006 Riisgaard-Pedersen published a study in Nature in which the NanoRespiration System was used for measuring denitrification by Nonionella cf. stella. based on N2O accumulation in the NanoRespiration Capillaries.

Ordering information

System components
Product
Sensor
OX-50 or other relevant microsensor
Amplifier
Microsensor Multimeter or another Unisense amplifier
NanoRespirometer
NanoRespirometer (7-capillaries, disc and frame)
Micromanipulator
MM33 or MM33-2 (Single or double head)
Motorized Microprofiling
MC-232 (Motor controller)
MMS (Micromanipulator stage)
MMS-A (Stage adapter)
Software
SensorTrace Profiling
Laboratory Stand
LS18
Calibration Chamber
CAL300

Related products

Oxygen Microsensor

Nielsen,P. et al (2007), Respiration rates of subitaneous eggs from a marine calanoid copepod: monitored by nanorespirometry, Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 287 - 296, vol. 177 Read abstract

Lopes,A.S. et al (2005), Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system, Reproduction, 669 - 679, vol. 130 Read abstract

Abe,H. (2007), A Non-invasive and Sensitive Method for Measuring Cellular Respiration with a Scanning Electrochemical Microscopy to Evaluate Embryo Quality, Journal of Mammalian Ova Research, 70 - 78, vol. 24

See all publications